Fig 1: LITAF/CDIP1 control the tubular recycling endosome. (Ai) Representative widefield images control or LITAF/CDIP1-depleted (KD) cells stained for Pacsin2 or for MICAL-L1. (Aii) Quantification of Pacsin2 and MICAL-L1 tubule density. Data combined from three independent experiments, total cell numbers shown in brackets. (Bi) Representative widefield images of control or LITAF/CDIP1-depleted HeLaM cells pulse-labelled with anti-MHC class I antibody or anti-CD59 antibody for 30 min at 37°C. Scale bar: 10 µm. (Bii) Quantitation of anti-MHC class I and anti-CD59-labelled tubule density. Quantitative results represented as Tukey box plot. P-values calculated with a Kolmogorov–Smirnov test. (C) Representative widefield images of control, LITAF/CDIP1-knockdown, or LITAF/CDIP1- and Rab11 co-depleted HeLaM cells, fixed and stained for Rab11. (D) Representative widefield images of control or LITAF/CDIP1-depleted cells stained for Pacsin2 and SNX1. (E) Representative EM images of control or LITAF/CDIP1-depleted cells. (F) Representative EM images of control or LITAF/CDIP1-depleted cells fixed in the presence of Ruthenium Red and processed for EM using conditions that minimally stained membranes that did not encounter ruthenium Red. Scale bars: 10 µm (A–D); 0.2 µm (E,F). Arrowheads highlight tubules.